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i-GTP

Anti-Gαi-GTP Mouse Monoclonal Antibody
5/5

¥4,800.00

货号:26901

规格: 30 微升

库存状态:In Stock

           产品描述          
产品名称:   i-GTP 小鼠单抗
货号:   26901
基因符号:   Gnai
描述:   i-GTP 小鼠单抗
背景:   Heterotrimeric G proteins are critical cellular signal transducers. Gαirepresents one sub-family of G proteins that could mediate the inhibition of adenylyl cyclases. Other biochemical and physiological functions of Gα i proteins are being explored.
免疫原:   Recombinant full length protein of active Gα i1
经过测试的应用:   IP, IHC and IF (Not applicable for WB since WB denatures the GTPase)
推荐稀释比:   IP: 1 µg for 1~2 mg total cellular proteinsIHC, IF: 1:50-1:250
Concentration:   1 mg/ml
种属反应性:   Mouse
形式:   Liquid
克隆性:   Monoclonal
亚型:   IgG1
纯化:   Purified from ascites
Preservative:  No
成分:  PBS (without Mg 2+ and Ca 2+ ), pH 7.4, 150 mM NaCl, 50% glycerol
种属反应性:  active Gα i antibody recognizes active Gα i1, Gα i2, and Gα i3 of vertebrates.
储存条件:   Store at -20°C. Avoid repeated freezing and thawing
免疫沉淀/蛋白质印迹:  i activation assay.A. CHO cells were transfected with wild type Gα i1 (lanes 1 and 2) or constitutively active Gα i1-Q204L (lane 3). Cell lysates were treated with GDP(lane 1) or GTPγS (lane 3). Lysates were then incubated with an anti-active Gα i monoclonal antibody (货号:26901) (top panel). The precipitated active Gα i was immunoblotted with an anti-Gα i monoclonal antibody (货号: 26003). The bottom panel shows the Western blot with anti-Gα i monoclonal antibody (货号: 26003) of the cell lysates.B. HEK293 cells stably expressing human m2 mAChR were treated with (lane 2) or without (lane 1)carbachol. Cell lysates were then incubated with an anti-active Gα i monoclonal antibody (货号: 26901) (top panel).The precipitated active Gα i was immunoblotted with an anti-Gα i 兔多抗 (货号: 21006). The bottom panel shows the Western blot with anti-tubulin of the cell lysates.
           引用文献          
01. Regulation of DNA damage response by trimeric G-proteins
iScience. 2023   PMID: 36756378
02. GPER mediates estrogen cardioprotection against epinephrine-induced stress
J Endocrinol. 2021  PMID: 33847279
03. Analgesic Efficacy of α2 Adrenergic Receptor Agonists Depends on the Chronic State of Neuropathic Pain: Role of Regulator of G Protein Signaling 4
Neuroscience. 2021  PMID: 33359660
04. Small Molecule Inhibitors Targeting Gαi2 Protein Attenuate Migration of Cancer Cells
Cancers. 2020  PMID: 32575572
05. Three-dimensional growth of breast cancer cells potentiates the anti-tumor effects of unacylated ghrelin and AZP-531
eLife. 2020  PMID: 32667883
06. Wnts regulate planar cell polarity via heterotrimeric G protein and PI3K signaling
J Cell Biol. 2020  PMID: 32805026
07. The COMMD3/8 complex determines GRK6 specificity for chemoattractant receptors
J Exp Med. 2019  PMID: 31088898
08. VLA-4 phosphorylation during tumor and immune cell migration relies on its coupling to VEGFR2 and CXCR4 by syndecan-1
J Cell Sci. 2019 2019  PMID: 31562188
09. Mechanoactivation of the angiotensin II type 1 receptor induces β‐arrestin‐biased signaling through Gαi coupling
J Cell Biochem. 2018  PMID: 29231251
10. Estrogen deficiency compromised the β 2 AR-Gs/Gi coupling: implications for arrhythmia and cardiac injury
Pflugers Arch. 2018  PMID: 29297096
11. Biased antagonism of CXCR4 avoids antagonist tolerance
Sci Signal. 2018   PMID: 30327409
12. Acupuncture Upregulates G Protein Coupled Activity in Samp8 Mice
Acupunct Med. 2017  PMID: 28450287
13. Gαi is required for carvedilol-induced β1 adrenergic receptor β-arrestin biased signaling
Nat Commun. 2017  PMID: 29167435
14. Sphingosine 1-phosphate (S1P) suppresses the collagen-induced activation of human platelets via S1P4 receptor
Thromb Res. 2017  PMID: 28609704
15. Wnt-5a/Frizzled9 Receptor Signaling through the Gαo-Gβγ Complex Regulates Dendritic Spine Formation
J Biol Chem. 2016  PMID: 27402827
16. Targeting brain tumor cAMP: the case for sex-specific therapeutics
Front. Pharmacol. 2015  PMID: 26283963
17. Rapid Actions of Xenoestrogens Disrupt Normal Estrogenic Signaling

Steroids. 2014  PMID: 24269739

18. The lipoprotein receptor LRP1 modulates sphingosine-1-phosphate signaling and is essential for vascular development
Development. 2014  PMID: 25377550
19. Neuronal NF1/RAS regulation of cyclic AMP requires atypical PKC activation
Hum Mol Genet. 2014  PMID: 25070947
20. Fatty Acid-binding Protein 5 (FABP5) Regulates Cognitive Function Both by Decreasing Anandamide Levels and by Activating the Nuclear Receptor Peroxisome Proliferator-activated Receptor ß/d (PPARß/d) in the Brain
J Biol Chem. 2014  PMID: 24644281
21. Structural basis for activation of trimeric Gi proteins by multiple growth factor receptors via GIV/Girdin
Mol Biol Cell. 2014  PMID: 25187647
22. Canonical and noncanonical g-protein signaling helps coordinate actin dynamics to promote macrophage phagocytosis of zymosan
Mol Cell Biol. 2014  PMID: 25225330
23. Identification of novel signalling roles and targets for Galpha and Gbetagamma downstream of the insulin-like growth factor 1 receptor in vascular smooth muscle cells
Biochem J. 2013  PMID: 23186281
24. Heterotrimeric Galpha(i) proteins are regulated by lipopolysaccharide and are anti-inflammatory in endotoxemia and polymicrobial sepsis
Biochim Biophys Acta. 2012  PMID: 21255617
25. CXCR4 activation defines a new subgroup of Sonic hedgehog-driven medulloblastoma
Cancer Res. 2012  PMID: 22052462
26. Regulation of Estradiol and Progesterone Production by CRH-R1 and -R2 Is through Divergent Signaling Pathways in Cultured Human Placental Trophoblasts
Endocrinology. 2012  PMID: 22865371
27. CRH activation of different signaling pathways results in differential calcium signaling in human pregnant myometrium before and during labor
J Clin Endocrinol Metab. 2012  PMID: 22869609
28. Estrogen- and xenoestrogen-induced ERK signaling in pituitary tumor cells involves estrogen receptor-à interactions with G protein-ài and caveolin I
Steroids. 2012  PMID: 22230296
29. WNT-5A stimulates the GDP/GTP exchange at pertussis toxin-sensitive heterotrimeric G proteins
Cell Signal. 2011  PMID: 21070854
30. Cannabinoids inhibit insulin receptor signaling in pancreatic β-cells
Diabetes.  2011  PMID: 21346174
31. PKA regulatory subunits mediate synergy among conserved G-protein-coupled receptor cascades
Nat Commun. 2011  PMID: 22186894
32. Na/H exchanger regulatory factors control parathyroid hormone receptor signaling by facilitating differential activation of G(alpha) protein subunits
J Biol Chem. 2010  PMID: 20562104
33. Beta-arrestin- but not G protein-mediated signaling by the “decoy” receptor CXCR7
Proc Natl Acad Sci U S A. 2010  PMID: 20018651