武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处


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cGMP EIA Kit – No Acetylation

Monoclonal cGMP EIA Kit - No Acetylation



规格: 96 well

库存状态:In Stock


Product Highlight

  • Based on the unique mouse monoclonal anti-cGMP antibody
  • The only assay kit on the market with monoclonal anti-cGMP antibody
    (all other kits are based on polyclonal anti-cGMP antibodies)
  • The monoclonal anti-cGMP antibody displays much higher selectivity over cAMP and GTP, than polyclonal anti-cGMP antibody
  • Avoids the batch-to-batch variations associated with polyclonal antibody productions from animals, thus providing the reproducibility

Product Description

Guanosine 3′, 5′-cyclic monophosphate (cyclic GMP; cGMP) plays critical regulatory roles in many physiological processes. cGMP is produced from GTP by guanylyl cyclases and is degraded by phosphodiesterases. Stimulation of guanylyl cyclases or inhibition of phosphodiesterases can increase cellular cGMP concentrations. Inhibitors of the cGMP-specific phosphodiesterases are used for treating human diseases. For example, inhibitors of cGMP specific phosphodiesterase type 5 (such as Viagra and Cialis) are used for treating erectile dysfunction.

To screen for inhibitors of phosphodiesterases or stimulators of guanylyl cyclases, it is essential to have a sensitive, selective and reproducible method to measure the cGMP concentrations. This is especially true for the initial screenings given the possible weaker effects of larger pools of compounds.

Currently available other ELISA kits measuring cGMP levels are based on the non-affinity-purified polyclonal anti-cGMP antibody. Despite the claimed selectivity, these polyclonal anti-cGMP antibodies display certain cross-reactivity with cAMP or GTP. In most cell types, cGMP is present at levels ~100 fold lower than cAMP.

NewEast Biosciences cGMP ELISA kit is based on the unique mouse monoclonal anti-cGMP antibody. This monoclonal anti-cGMP antibody displays >108 fold of selectivity over cAMP, GTP and other nucleoside analogues. NewEast Biosciences cGMP ELISA kit provides significantly improved sensitivity and selectivity over other kits based on polyclonal anti-cGMP antibodies. Our monoclonal anti-cGMP antibody-based ELISA kit also avoids the batch-to-batch variations associated with polyclonal antibody productions from animals, thus providing the reproducibility in the long run.

Principle Outline

NewEast Biosciences cGMP ELISA Kit is a competitive immunoassay to measure the cGMP levels, either from cell extracts or from in vitro guanylyl cyclase assays. Briefly, multi-well plates are coated with goat-anti-mouse serum. cGMP in cell extracts or in in vitro guanylyl cyclase assays will competitively bind to the monoclonal anti-cGMP antibody in the presence of fixed amounts of cGMP-conjugated horse-radish peroxidase or alkaline phosphatase. Known amounts of cGMP are used as standards to generate the calculation curve. After a short incubation, the excess reagents are washed away and substrate is added. The multiwell plates are then read on a microplate reader at 450 nm or 405 nm. The intensity of the yellow color is inversely proportional to the concentration of cGMP in samples. The measured optical density is used to calculate the concentration of cGMP in samples based on the curve from the cGMP standards.

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Int J Med Mushrooms. 2020   PMID: 33426827
02. Phosphodiesterase 10A (PDE10A) as a novel target to suppress β-catenin and RAS signaling in epithelial ovarian cancer
J Ovarian Res. 2022
03. Inhibition of Hypoxia-Inducible Factor Prolyl-Hydroxylase Modulates Platelet Function
Thromb Haemost. 2022
04. PDGF regulates guanylate cyclase expression and cGMP signaling in vascular smooth muscle
Commun Biol., 2022
05. Celecoxib reduces hepatic vascular resistance in portal hypertension by amelioration of endothelial oxidative stress
J Cell Mol Med. 2021
06. Matrine Impairs Platelet Function and Thrombosis and Inhibits ROS Production
Front Pharmacol. 2021
07. Theacrine, a Potent Antidepressant Purine Alkaloid from a Special Chinese Tea, Promotes Adult Hippocampal Neurogenesis in Stressed Mice
J Agric Food Chem. 2021
08. Homogeneous single-label cGMP detection platform for the functional study of nitric oxide-sensitive (soluble) guanylyl cyclases and cGMP-specific phosphodiesterases
Sci Rep., 2020
09. 17β-Estradiol nongenomically induces vascular endothelial H2S release by promoting phosphorylation of cystathionine γ-lyase
J Biol Chem. 2019
10. Is NO the Answer? The Nitric Oxide Pathway Can Support Bone Morphogenetic Protein 2 Mediated Signaling
Cells. 2019
11. DHA induces apoptosis of human malignant breast cancer tissues by the TLR-4/PPAR-α pathways
Oncol Lett. 2018
12. Ferulic acid induces proliferation and differentiation of rat osteoblasts in vitro through cGMP/PKGII/ENaC signaling
Oncol Lett. 2018
13. Natriuretic peptide receptor 2 (NPR2) localized in bovine oocyte underlies a unique mechanism for C-type natriuretic peptide (CNP)-induced meiotic arrest
Theriogenology. 2018
14. Interplay between Obesity-Induced Inflammation and cGMP Signaling in White Adipose Tissue
Cell Rep. 2017
15. N-octanoylated ghrelin peptide inhibits bovine oocyte meiotic resumption
J Asian Nat Prod Res., 2017
16. Novel effect of Daflon and low-dose γ-radiation in modulation of thioacetamide-induced hepatic encephalopathy in male albino rats
Hum Exp Toxicol. 2017
17. Sulfhydration-associated phosphodiesterase 5A dimerization mediates vasorelaxant effect of hydrogen sulfide
Oncotarget. 2017
18. Characterization and expression of soluble guanylate cyclase in skins and melanocytes of sheep
Acta Histochem. 2016
19. Cross regulation between cGMP-dependent protein kinase and Akt in vasodilatation of porcine pulmonary artery
J Control Release. 2016
20. Improvement of vascular function by magnetic nanoparticle-assisted circumferential gene transfer into the native endothelium
Am J Physiol Regul Integr Comp Physiol. 2016
21. The vasodilatory effect of sulfur dioxide via SGC/cGMP/PKG pathway in association with sulfhydryl-dependent dimerization
Am J Physiol Regul Integr Comp Physiol. 2016
22. Chronic β1-adrenergic blockade enhances myocardial β3-adrenergic coupling with nitric oxide-cGMP signaling in a canine model of chronic volume overload: new insight into mechanisms of cardiac benefit with selective β1-blocker therapy
Basic Res Cardiol. 2015
23. Identification of a novel microRNA important for melanogenesis in alpaca (Vicugna pacos)
J Anim Sci. 2015
24. Alteration of vascular reactivity in heart failure: role of phosphodiesterases 3 and 4
Br J Pharmacol., 2014
25. Genetic ablation of solute carrier family 7a3a leads to hepatic steatosis in zebrafish during fasting
Hepatology, 2014
26. Preservation of nitric oxide-induced relaxation of porcine coronary artery: roles of the dimers of soluble guanylyl cyclase, phosphodiesterase type 5, and cGMP-dependent protein kinase
Pflugers Arch. 2014
27. The effects of fusion structure on the expression and bioactivity of human brain natriuretic peptide (BNP) albumin fusion proteins
Curr Pharm Biotechnol. 2014
28. Defining Specificity Determinants of Cyclic GMP-Mediated Gustatory Sensory Transduction in Caenorhabditis elegans
Genetics. 2013
29. Sulfhydryl-dependent dimerization of soluble guanylyl cyclase modulates the relaxation of porcine pulmonary arteries to nitric oxide
Pflugers Arch. 2013
30. The role of nitric oxide signaling in food intake; insights from the inner mitochondrial membrane peptidase 2 mutant mice
Redox Biology, 2013
31. The Soluble Guanylate Cyclase Activator BAY 58-2667 Protects against Morbidity and Mortality in Endotoxic Shock by Recoupling Organ Systems
PLoS One. 2013
32. Volume overload induces differential spatiotemporal regulation of myocardial soluble guanylyl cyclase in eccentric hypertrophy and heart failure
Journal of Molecular and Cellular Cardiology, 2013
33. Heterogeneity in relaxation of different sized porcine coronary arteries to nitrovasodilators: role of PKG and MYPT1
Pflugers Arch. 2012
34. Hypoxia induces downregulation of soluble guanylyl cyclase β1 by miR-34c-5p
J Cell Sci. 2012
35. Inhibition of phosphoinositide 3-kinase potentiates relaxation of porcine coronary arteries induced by nitroglycerin by decreasing phosphodiesterase type 5 activity
Circ J. 2012
36. Natriuretic peptides block synaptic transmission by activating phosphodiesterase 2A and reducing presynaptic PKA activity
Proc Natl Acad Sci U S A. 2012
37. Pressure-overload-induced subcellular relocalization/oxidation of soluble guanylyl cyclase in the heart modulates enzyme stimulation
Circ Res. 2012
38. Inhibition of Striatal Soluble Guanylyl Cyclase-cGMP Signaling Reverses Basal Ganglia Dysfunction and Akinesia in Experimental Parkinsonism
PLoS One. 2011
39. Molecular analysis, developmental function and heavy metal-induced expression of ABCC5 in zebrafish
Comp Biochem Physiol B Biochem Mol Biol. 2011
40. Molt-inhibiting hormone from Chinese mitten crab (Eriocheir sinensis): Cloning, tissue expression and effects of recombinant peptide on ecdysteroid secretion of YOs
Gen Comp Endocrinol. 2011
41. Role of sulfhydryl-dependent dimerization of soluble guanylyl cyclase in relaxation of porcine coronary artery to nitric oxide
Cardiovasc Res. 2011
42. Feedback control through cGMP-dependent protein kinase contributes to differential regulation and compartmentation of cGMP in rat cardiac myocytes
Circ Res. 2010
43. Stimulation of guanylyl cyclase-D by bicarbonate
Biochemistry. 2009