武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处

邮箱:info@neweastwuhan.com

咨询:027 – 63497286 

E. coli Protein Service

E coli Protein Service

We offer multiple fusion/affinity tags to increase the protein solubility, expression levels, and ease of purification.System

The advantages of E. coli expression systems include easy operation, low cost, high expression, as well as in-depth research on its genetic background and the diversity of vector strain selection, the use of E. coli to overexpress target genes is still the preferred method at present. However, the codon preferences of different species and the cytotoxicity and solubility of expressed products affect the expression of heterologous genes. With our rich experience, We offer multiple fusion/affinity tags to increase the protein solubility, expression levels, and ease of purification. 

HighlightsDetails
High Success RateSuccess rate of the soluble protein expression provided using E. coli can be up to 95%.
Optional Expression VectorsNewEast provides a variety of screened and reformed E. coli expression vectors to meet the specific requirements of intracellular and periplasmic; Space secretory expression including the pET series, the pBAD series, the pGEX series; The pCold series.
Optional Protein TagsWe use solubilized tags such as SUMO, Trx, GST, and NusA to improve; The hydrophilicity of protein in inclusion body expression.
Optional Expression HostsNewEast can reform and use more than 10 E. coli expression hosts; Including the standard expression strain, the protein expression enhanced strain, the toxic Protein expression strain, the ultra-low temperature strain for enhancing soluble protein expression, and the other expression strains, to meet the customers’ different requirements.
Large-scale Production1-4,000 L per batch.
One-stop Service From design and optimize gene, optimize protein expression conditions, purify protein and post processing, to managing of data and report.
StepDescriptionTimeline
Plasmid ConstructionGene synthesis with codon optimization & subclone2 Weeks
Protein Expression TestPlasmid transformation
Exploration of expression condition, expression analysis and identification (SDS-PAGE and/or WB)
200 mL amplification in optimum condition and purification test
3 Weeks
Large-Scale Fermentation & PurificationScale up protein expression using optimized conditions
Protein affinity purification (desired protein amounts and purity)
Denaturation and renaturation of inclusion bodies (if required)
Tag-free protein (if required), endotoxin removing (if required)
Quality control
3-5 Weeks
Mammalian Protein Service

Mammalian Protein Expression Service

We are an expert on CHO and HEK293 cell expression.  We can meet your special needs such as serum-free culture, tag excision, endotoxin removal, and mass spectrometry analysis.
Mammalian cells have become the preferred host cells in the field of biopharmaceuticals. Mammalian cell expression systems can introduce proper protein folding  and carry out post-translational modifications, which are essential for full biological activity. The expressed recombinant proteins are closest to the human conformation and have the same biological activity as natural proteins. Heterologous proteins expressed by mammalian cells have the incomparable advantages of specific prokaryotic expression systems and eukaryotic expression systems such as yeast and insect cells in terms of activity, which are closer to natural proteins. Therefore, mammalian cell expression systems have been widely used in the development and production of therapeutic recombinant monoclonal antibody drugs and protein drugs. We has established a mature mammalian protein expression system and purification service platform, providing expression and purification services of mammalian protein expression system to help you quickly obtain high-quality protein.
Advantage
   
HighlightsSummary
High activity and high success rateProteins expressed in mammalian cells can achieve complex post-translational modifications.
Multiple expression system options Transient transfection expression
Stable transfection expression
Recombinant antibody expression,and preparation
One-stop Service Design and optimize gene
Optimize protein expression conditions
Purify protein and post processing
Manage data and report.
Stable Cell Line Construction
   
StepDescriptionTimeline
Vector DesignConducts bioinformatic analysis on target(s) and recommends an expression strategy to each client.1 Week
Plasmid ConstructionGene optimization and synthesis, Vector construction2-3 Weeks
Identification of polyclonal stable cell linesVector transformation, screening and identification
identification of positive cell clones
2-3 Weeks
Vector transformation, screening and identification
identification of positive cell clones
Cell subclonal screening to screen out monoclonal cell lines with high expression levels and stable performance3-4 Weeks
Stable cell line quality testPerform performance verification according to customer requirements, such as protein activity, expression level, cell passage stability, etc.1-2 Weeks
Yeast Protein Services

Yeast Protein Expression Services

Yeast expression system is one of the most economical eukaryotic expression system for scale up production of both secreted and intracellular proteins that require post-translational modifications

Yeast protein expression system is the most economical and efficient expression system among eukaryotic protein expression systems, which combines the advantages of prokaryotic expression system and eukaryotic expression system. Because of its fast growth rate, low culture cost and clear genetic background, it has been widely used in industrial microorganisms. The yeast expression system can correctly process, modify and fold post-translational proteins, so it is often used for the expression of heterologous functional proteins in eukaryotes. Among them, the Pichia pastoris expression system is more widely used. Compared with other systems, it has unique advantages, such as strong promoters FLD1, GAP, AOX1, etc., which can make the expression of heterologous proteins lasting and efficient.

Advantage

HighlightsSummary
High activity and high success rateAccording to the codon preference, to optimize the target gene, Effectively improve protein solubility and expression
Multi-carrier selection schemeAccording to the characteristics of different proteins Vectors suitable for extracellular secretion expression can be provided, vectors that can also be expressed intracellularly
Optimization of induction conditions By optimization of Conditions such as methanol concentration and induction time , we can effectively improve the expression of protein.
Extra ServicesTag removal
Endotoxin removal

Stable Cell Line Construction Service

Process StepDescriptionTimeline
Vector DesignConducts bioinformatic analysis on target(s) and recommends an expression strategy to each client.1 Week
Plasmid ConstructionGene optimization and synthesis, Vector construction2-3 Weeks
Identification of polyclonal stable cell lines Vector transformation, screening and identification
identification of positive cell clones
2-3 Weeks
Screening of monoclonal stable cell lines Cell subclonal screening to screen out monoclonal cell lines with high expression levels and stable performance3-4 Weeks
Stable cell line quality testPerform performance verification according to customer requirements, such as protein activity, expression level, cell passage stability, etc.1-2 Weeks