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HELT Rabbit pAb

Anti-HELT Rabbit Polyclonal Antibody
5/5

¥2,850.00

货号:S222174

规格: 100 微升

库存状态:In Stock

           产品描述          
货号:   S222174
产品全名:   HELT 兔多抗
基因符号   Mgn; HESL; HCM1228; bHLHb44
UNIPROT ID:   A6NFD8 (Gene Accession – NP_001287710 )
背景:   Transcriptional repressor which binds preferentially to the canonical E box sequence 5′-CACGCG-3′.
抗原:   Synthetic peptide of human HELT
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   IHC: 20-100;WB: 200-1000;ELISA: 5000-10000
种属反应性:   Rabbit
克隆性:   Rabbit Polyclonal
亚型:   Immunogen-specific rabbit IgG
纯化:   Antigen affinity purification
种属反应性:   Human, Mouse
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
研究领域:   Epigenetics and Nuclear Signaling
储存和运输:   Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human esophagus cancer tissue using 222174(HELT Antibody) at a dilution of 1/30(Nucleus or Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with the synthetic peptide and then with 222174(Anti-HELT Antibody) at dilution 1/30.

The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using 222174(Anti-HELT Antibody) at a dilution of 1/30.

In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with synthetic peptide and then with D264169(Anti-HELT Antibody) at dilution 1/30.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: Mouse liver tissue, Mouse heart tissue and Mouse brain tissue lysates;
Primary antibody: 222174(HELT Antibody) at dilution 1/350;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 5 minutes
           引用文献