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Arl1-GTP

Anti-Arl1-GTP Mouse Monoclonal Antibody
5/5

¥4,800.00

货号:26924

规格: 30 微升

库存状态:In Stock

           产品描述          
产品名称:   Arl1-GTP 小鼠单抗
货号:   货号:: 26924
规格:   30 µL
基因符号:   Arl1
描述:   Arl1-GTP 小鼠单抗
背景:   Arl1 (Arf-like 1) localizes to the Golgi complex, where it is believed to recruit effector proteins to the trans-Golgi network. Golgi-localized proteins golgin-97 and golgin-245 have been shown as Arl1 effectors. Additional Arl1 effectors include the GARP (Golgi-associated retrograde protein)/VFT (Vps53) vesicle-tethering complex and Arfaptin 2. In Drosophila zygotes, mutation of Arl1 is lethal.
免疫原 :   Recombinant Arl1 protein (active form)
经过测试的应用:   IP, IHC and IF (Not applicable for WB since WB denatures the GTPase)
推荐稀释比:   
IP: 1 µg for 1~2 mg total cellular proteins
IHC, IF: 1:50-1:250
Concentration:   1 mg/ml
种属反应性:   Mouse
形式:   Liquid
克隆性:  Monoclonal
亚型:   IgG
纯化:   Purified from ascites
Preservative:  No
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
种属反应性:   Arl1 antibody recognizes active Arl1-GTP from vertebrates.
储存条件:   Store at -20°C. Avoid repeated freezing and thawing

免疫沉淀/蛋白质印迹:   
Arl1 Activation Assay.

Purified GST-tagged Arl1 proteins (货号:10136) were immunoprecipitated with the anti-active Arl1 monoclonal antibody (货号:   26924) after treated with GDP (lane 1) or GTPγS (lane 2), and was blotted with anti- Arl1 monoclonal antibody (货号:26056). Arl1 protein input control is shown in the bottom panel.

           引用文献