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β-Catenin pAb

Anti-β-catenin Rabbit Polyclonal Antibody
5/5

¥2,600.00

货号:21282

规格: 100 微升

库存状态:In Stock

           产品描述          
产品名称:   β-Catenin 兔多抗
货号:   21282
基因符号:   CTNNB1, CTNNB
描述:   β-catenin 兔多抗
背景:   Beta-catenin (or β-catenin) is a protein that in humans is encoded by the CTNNB1 gene. β-catenin is a subunit of the cadherin protein complex and also acts as an intracellular signal transducer in the Wnt signaling pathway. Deregulation of beta-catenin signaling is an important event in the genesis of a number of malignancies, such as colon cancer, melanoma, hepatocellular carcinoma, ovarian cancer, endometrial cancer, medulloblastoma pilomatricomas, and prostate cancer.
免疫原 :    A synthetic peptide from the internal region of β-catenin.
经过测试的应用:   ELISA, WB, IF, IHC
推荐稀释比:   
ELISA: 1:1000-1:5000
WB: 1:500-1:2000
IHC: 1:50-1:100
Concentration:   1 mg/ml
种属反应性:   Rabbit
形式:   Liquid
克隆性:  Polyclonal
亚型:   IgG
纯化:    Purified from serum
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
种属反应性:   Recognizes β-catenin from vertebrates.
储存条件:    Store at -20°C. Avoid repeated freezing and thawing

蛋白质印迹:   

Western blot analysis of recombinant β-catenin protein. Purified His-tagged β-catenin protein (amino acids 1-76) was blotted with anti- β-catenin 兔多抗 (货号:21282).
Immunohistochemistry:   

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with anti-β-catenin 兔多抗 (货号:21282). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:50 dilution) overnight at 4°C. A HRP-conjugated Goat anti-mouse IgG (1:50 dilution) was used as secondary antibody.
           引用文献