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β-Catenin(E53K)

Anti-β-catenin(E53K) Mouse Monoclonal Antibody
5/5

¥4,800.00

货号:26167

规格: 100 微升

库存状态:In Stock

           产品描述          
产品名称:   β-Catenin(E53K) 小鼠单抗
货号:   26167
基因符号:   CTNNB1, CTNNB
描述:   β-catenin(E53K) 小鼠单抗
背景:   Beta-catenin (or β-catenin) is a protein that in humans is encoded by the CTNNB1 gene. β-catenin is a subunit of the cadherin protein complex and also acts as an intracellular signal transducer in the Wnt signaling pathway. Deregulation of beta-catenin signaling is an important event in the genesis of a number of malignancies, such as colon cancer, melanoma, hepatocellular carcinoma, ovarian cancer, endometrial cancer, medulloblastoma pilomatricomas, and prostate cancer.
免疫原:   A synthetic peptide from the internal region of β-catenin which includes the mutation of E53K, human origin.
经过测试的应用:   ELISA, WB, IF, IHC
推荐稀释比:   ELISA: 1:1000-1:5000WB: 1:500-1:2000IHC: 1:50-1:100
Concentration:   1 mg/ml
种属反应性:   Mouse
形式:   Liquid
克隆性:   Monoclonal
亚型:   IgG
纯化:   Purified from ascites
成分:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
种属反应性:   Recognizes β-catenin(E53K), but not wild type β-catenin protein from vertebrates.
储存条件:    Store at -20°C. Avoid repeated freezing and thawing
蛋白质印迹:  Western blot analysis of recombinant β-catenin(E53K) and wild type proteins. Purified His-tagged β-catenin(E53K) protein (amino acids 1-76, lane 2) and corresponding wild type protein (lane 1) were blotted with Anti-β-catenin(E53K) 小鼠单抗 (货号:26167).Immunofluorescence:  Immunofluorescence of cells expressing β-catenin proteins with anti-β-catenin(E53K) antibody. HEK293T cells were transfected with pCDNA3-GFP- β-catenin (WT) plasmid (left column) or pCDNA3-GFP-β-catenin(E53K) plasmid (right column), then fixed and stained with Anti-β-catenin (E53K) monoclonal antibody (货号:26167).
           引用文献