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ERBB2 pAb

Anti-ERBB2 Rabbit Polyclonal Antibody
5/5

¥2,600.00

货号:21202

规格: 100 微升

库存状态:In Stock

           产品描述          
产品名称:   ErbB2 兔多抗
货号:   21202
基因符号:   ERBB2/HER2/Neu
描述:   ErbB2 兔多抗
背景:   The ErbB family is composed of four plasma membrane-bound receptor tyrosine kinases. Amplification or over-expression of the ERBB2 gene occurs in approximately 30% of breast cancers. HER2 is encoded by ERBB2, a known proto-oncogene located at the long arm of human chromosome 17(17q21-q22). HER2 proteins have been shown to form clusters in cell membranes that may play a role in tumorigenesis.
免疫原 :   A synthetic peptide from the central of ErbB2 protein.
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   
ELISA: 1:500-1:5000
WB: 1:200-1:2000
IHC: 1:20-1:200
Concentration:   0.2 mg/ml
种属反应性:   Rabbit
形式:   Liquid
克隆性:  Polyclonal
亚型:   IgG
纯化:   Purified from serum
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
种属反应性:   Recognizes ErbB2 of vertebrates.
储存条件:   Store at -20°C. Avoid repeated freezing and thawing.
蛋白质印迹:   

Western blot analysis of ErbB2 protein. Purified full length His-tagged ErbB2 protein (货号:10208) was blotted with Anti-ErbB2 兔多抗 (货号:21202).

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Anti-ErbB2 兔多抗 (货号:21202). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:50 dilution) overnight at 4 °C. A HRP-conjugated Goat anti-Rabbit IgG (1:50) was used as secondary antibody.
           引用文献