武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处

邮箱:info@neweastwuhan.com

咨询:027 – 63497286 

s-GTP

Anti-Gαs-GTP Mouse Monoclonal Antibody
5/5

¥4,800.00

货号:26906

规格: 30 微升

库存状态:In Stock

           产品描述          
产品名称:   s-GTP 小鼠单抗
货号:   26906
Size: </strong  30 µL>
基因符号:  Gnas
描述:  Anti-Gαs-GTP 小鼠单抗
背景:   Heterotrimeric G proteins are critical cellular signal transducers. Gαsrepresents one sub-family of G proteins that could mediate the stimulation of adenylyl cyclases.
Immunogen:  Recombinant full length protein of active Gαs
经过测试的应用:  IP, IHC and IF (Not applicable for WB since WB denatures the GTPase)
推荐稀释比:  IP: 1 µg for 1~2 mg total cellular proteinsIHC, IF: 1:50-1:250
Concentration:   1 mg/ml
种属反应性: </strong  Mouse>
形式:   Liquid
克隆性:   Monoclonal
亚型:  IgG
纯化:  Purified from ascites
成分:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
种属反应性:   Anti-active Gαs antibody recognizes active Gαs of vertebrates
储存条件:     Store at -20°C. Avoid repeated freezing and thawing
免疫沉淀/蛋白质印迹:   s Activation Assay. Purified Gαs proteins were loaded as a control (lanes 1) or immunoprecipitated after treated with GDP (lane 2) or GTPγS (lane 3). Immunoprecipitation was done with the anti Gαs-GTP monoclonal antibody (货号: 26906). Immunoblot was with an anti- Gαsmonoclonal antibody (货号: 26006).
           引用文献          
01. TNF-α impairs EP4 signaling through the association of TRAF2-GRK2 in primary fibroblast-like synoviocytes
Acta Pharmacol Sin. 2022   PMID: 33859345
02. Identification of expression and function of the glucagon-like peptide-1 receptor in colonic smooth muscle
Peptides. 2019  PMID: 30508636
03. Estrogen deficiency compromised the á 2 AR-Gs/Gi coupling: implications for arrhythmia and cardiac injury
Pflugers Arch. 2018  PMID: 28609704
04. Polycystin 1 loss of function is directly linked to an imbalance in G-protein signaling in the kidney
Development. 2018  PMID: 29530879
05. Acupuncture Upregulates G Protein Coupled Activity in Samp8 Mice
Acupunct Med. 2017  PMID: 28450287
06. Follicular Stimulating Hormone Accelerates Atherogenesis by Increasing Endothelial VCAM-1 Expression
Theranostics. 2017  PMID: 29187895
07. Nuclear translocation of nuclear factor kappa B is regulated by G protein signaling pathway in arsenite?induced apoptosis in HBE cell line
Environmental toxicology. 2016  PMID: 26306706
08.  Neuronal NF1/RAS regulation of cyclic AMP requires atypical PKC activation
Hum. Mol. Genet. 2014  PMID: 25070947
09. Rapid Actions of Xenoestrogens Disrupt Normal Estrogenic Signaling
Steroids. 2014  PMID: 24269739
10. The G-protein Alpha Subunit Gsa Is A Tumor Suppressor In Sonic Hedgehog-driven Medulloblastoma
Nat Med. 2014  PMID: 25150496
11. Corticotropin-releasing hormone stimulates mitotic kinesin-like protein 1 expression via a PLC/PKC-dependent signaling pathway in hippocampal neurons
Mol Cell Endocrinol. 2012  PMID: 22698524
12. CRH activation of different signaling pathways results in differential calcium signaling in human pregnant myometrium before and during labor
J Clin Endocrinol Metab. 2012  PMID: 22869609
13. Estrogen- and xenoestrogen-induced ERK signaling in pituitary tumor cells involves estrogen receptor-a interactions with G protein-ai and caveolin I
Steroids. 2012  PMID: 22230296
14. Estrogenic action on arterial smooth muscle: permissive for maintenance of CRHR2 expression
Endocrinology. 2012  PMID: 22315451
15. Glucocorticoid acts on a putative G protein-coupled receptor to rapidly regulate the activity of NMDA receptors in hippocampal neurons
Am J Physiol Endocrinol Metab. 2012  PMID: 22146309
16. Insulin-like growth factor effects on vascular smooth muscle cells are in part modulated via a G protein-coupled pathway
University of Manitoba. 2010