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Rab3A(Q81L) Protein

Rab3A Protein Q81L mutant
5/5

¥347.00

货号:10129

规格: 25 微升

库存状态:In Stock

           产品描述          
产品名称:   Rab3A(Q81L) 突变蛋白
货号:   10129
产品全名:   Rab3A Protein Q81L 突变蛋白
基因符号  Member RAS oncogene family
Source:   Human, recombinant full length, His6-tag
Expression 种属反应性:   E. coli
分子量:   25 kDa
纯化:   >95% by SDS-PAGE
Introduction:   The Rab3 small G protein family consists of four members, Rab3A, -3B, -3C, and -3D. Rab3A is found specifically in brain and regulates a late step in synaptic vesicle fusion. Rab3A -mediated synaptic transmission is involved in circadian period and sleep homeostasis.
Amino Acid Sequence  (1-220, Q81L)
MASATDSRYGQKESSDQNFDYMFKILIIGNSSVGKTSFLFRYADDSFTPAFVSTVGIDFKVKTIYRN
DKRIKLQIWDTAGLERYRTITTAYYRGAMGFILMYDITNEESFNAVQDWSTQIKTYSWDNAQVLLVG
NKCDMEDERVVSSERGRQLADHLGFEFFEASAKDNINVKQTFERLVDVICEKMSESLDTADPAVTGAKQ
GPQLSDQQVPPHQDCAC
Properties
Physical Appearance (form):   Dissolved in 20mM Tris-HCl, pH8.0, 150mM NaCl.
Physical Appearance (form):   White or clear
Concentration:1 mg/mL
Storage:    -80°C
Preparation Instructions:   
Centrifuge the vial before open the cap and reconstitute in water. Adding of 10 mM β-mercaptoethanol or 1 mM DTT into the solution to protect the protein is recommended and using of non-ionic detergents such as n-Dodecyl β-D-maltoside (DoDM) or polyethylene detergents (e.g. C12E10) also help to stabilize the protein. Avoid repeated freezing and thawing after reconstitution. The purity of His-tagged Rab3A Q81L was determined by SDS-PAGE and Coomassie Brilliant Blue Staining.


References:   
1. Geppert, M. et al., Nature 387: 810-814, 1997.
2. Giovedi, S. et al., J. Biol. Chem. 279: 43769-43779, 2004.
3. Huang, Y.-Y. et al., Proc. Nat. Acad. Sci. 102: 9365-9370, 2005.
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8. Trask, B. et al., Genomics 15: 133-145, 1993.
9. Zahraoui, A. et al., J. Biol. Chem. 264: 12394-12401, 1989.

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