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BRaf pAb

Anti-BRaf Rabbit Polyclonal Antibody
5/5

¥2,600.00

货号:21271

规格: 100 微升

库存状态:In Stock

           产品描述          
产品名称:   BRaf 兔多抗
货号:   21271
基因符号:   B-RAF, B-RAF1, RAFB1
描述:   BRaf 兔多抗
背景:   B-Raf is a member of the Raf family of Ser/Thr protein kinases. It functions downstream of Ras to regulate the MAP kinase signaling pathway. Mutations in the B-RAF gene cause diseases. Inherited mutations in B-RAF cause cardiofaciocutaneous syndrome. Acquired mutations in B-RAF have been found in cancers.
免疫原 :   Purified B-Raf protein, human origin.
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   
ELISA: 1:1000-1:5000
WB: 1:100-1:1000
IHC: 1:50-1:200
Concentration:    0.1mg/ml
种属反应性:   Rabbit
形式:   Liquid
克隆性:  Polyclonal
亚型:   
纯化:   Purified from serum
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
种属反应性:   Recognizes B-Raf of vertebrates.
储存条件:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry:   

Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue with anti- B-Raf 兔多抗 (货号:21271). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:50 dilution) overnight at 4°C. A HRP-conjugated Goat anti-mouse IgG (1:50 dilution) was used as secondary antibody.