武汉费斯德生物科技有限公司, NewEast Biosciences 中国办事处

邮箱:info@neweastwuhan.com

咨询:027 – 63497286 

o-GTP

Anti-Gαo-GTP Mouse Monoclonal Antibody
5/5

¥4,800.00

货号:26907

规格: 30 微升

库存状态:In Stock

           产品描述          
产品名称:   o-GTP 小鼠单抗
货号:   26907
规格:   30 µL
基因符号:   Gnao
描述:   Anti  Gαo-GTP 小鼠单抗
背景:   Heterotrimeric G proteins are critical cellular signal transducers. Gαorepresents one sub-family of G proteins that could mediate the inhibition of adenylyl cyclases. Other biochemical and physiological functions of Gαo proteins are being explored.
免疫原:   Recombinant full length protein of active Gαo
经过测试的应用:   IP, IHC and IF (Not applicable for WB since WB denatures the GTPase)
推荐稀释比:   IP: 1 µg for 1~2 mg total cellular proteinsIHC, IF: 1:50-1:250
Concentration:   1 mg/ml
种属反应性:   Mouse
形式:   Liquid
克隆性:   Monoclonal
亚型:   IgG1
纯化:   Purified from ascites
Preservative:  No
成分:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
种属反应性:  active Gαo antibody recognizes active Gαo of vertebrates.
储存条件:   Store at -20°C. Avoid repeated freezing and thawing
免疫沉淀/蛋白质印迹:  o Activation Assay.
Purified Gαo proteins were loaded as a control (lanes 1) or immunoprecipitated after treated with GDP (lane 2) or GTPγS (lane 3). Immunoprecipitation was done with the anti-active Gαo monoclonal antibody (货号: 26907). Immunoblot was with an anti-Gαo兔多抗 (货号: 21015).