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HRAS(G13A)

Anti-HRAS(G13A) Mouse Monoclonal Antibody
5/5

¥4,800.00

货号:26191

规格: 100 微升

库存状态:In Stock

           产品描述          
产品名称:   Ras(G13A) 
货号:   26191
基因符号:   H-Ras; K-Ras; N-Ras
描述:   HRAS(G13A) 小鼠单抗
背景:   Belongs to the small GTPase superfamily. Ras family binds GDP/GTP and possess intrinsic GTPase activity, alternate between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP).Located in cell membrane, lipid-anchor, cytoplasmic side and Golgi apparatus membrane.
免疫原:   A synthetic peptide from the internal region of HRas which includes the mutation of G13A, human origin.
经过测试的应用:   ELISA, WB, IHC
推荐稀释比:   ELISA: 1:1000-1:5000WB: 1:500-1:1000IHC: 1:50-1:100
Concentration:   1 mg/ml
种属反应性:   Mouse
形式:   Liquid
克隆性:   Monoclonal
亚型:   IgG
纯化:   Purified from ascites
成分:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
种属反应性:  Recognizes G13A mutant, but not wild type H-RAS of vertebrates.
储存条件:    Store at -20°C. Avoid repeated freezing and thawing
蛋白质印迹:  Western blot analysis of recombinant HRAS(G13A) and wild type proteins.Purified His-tagged HRAS(G13A) protein (lane 2) and corresponding wild type protein (lane 1) were blotted with Anti-HRAS(G13A) monoclonal antibody (货号:26191).Immunofluorescence:   Immunofluorescence of cells expressing Ras proteins with Anti-HRAS(G13A) antibody. HEK293T cells were transfected with pCDNA3-GFP-HRas (WT) plasmid (left column) or pCDNA3-GFP-HRAS(G13A) plasmid (right column), then fixed and stained with Anti-HRAS(G13A) monoclonal antibody (货号:26191).